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MycoTest.

Mushroom potency analysis: laboratory quantification of tryptamine alkaloids

MycoTest is MycoBag’s laboratory analysis service for mycology research. Send a dried sample to our laboratory and receive a quantitative tryptamine profile – psilocybin, PCBE (psilocybin + psilocin) and total alkaloids – by email in less than 3 weeks, for €25 per sample.

€25per sample
< 3 weeksto your report
3 valuespsilocybin · PCBE · total alkaloids
Confidentialcoded, anonymous samples

How MycoTest works

Illustration of ordering MycoTest online on a computer

Order your analysis

Purchase a MycoTest online. Each analysis corresponds to one sample and one unique identification code.

Illustration of registering the MycoTest code on the package

Label your sample

Write your unique code on the envelope included in your MycoTest Bag. This code links the sample to its report and keeps the process anonymous and traceable.

Illustration of a mushroom sample placed into the MycoTest bag

Prepare your sample

Place the fully dried sample in the MycoTest Bag and seal it. The packaging limits exposure to air, light and physical damage during transit.

Illustration of hands packing a MycoTest sample for shipping

Send it to the laboratory

Post the pre-printed envelope – no address needed. Once received, the sample is registered under its code and queued for analysis.

Illustration of the MycoTest report received on a tablet

Receive your report

Within 3 weeks you receive a quantitative report by email with three values: psilocybin, PCBE (psilocybin + psilocin) and total alkaloids.

Quantitative results by email in less than 3 weeks

Accessible laboratory data

Analytical testing is often expensive and hard to access for independent researchers. MycoTest makes it simple: a quantitative tryptamine profile for €25 per sample, delivered by email in less than 3 weeks. Affordable enough to test every strain, flush or drying method you want to compare.

MycoTest psilocybin analysis report displayed on a tablet

What does MycoTest measure?

The main bioactive compounds in Psilocybe species are indole alkaloids of the tryptamine family. Their concentration cannot be estimated from size, colour or morphology: it has to be quantified analytically. Each MycoTest report includes three values:

  • Psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine) – the phosphorylated tryptamine that is usually the most abundant alkaloid in the fruiting body. It is relatively stable once the material is properly dried.
  • PCBE (psilocybin + psilocin) – the combined content of psilocybin and its dephosphorylated form, psilocin (4-hydroxy-N,N-dimethyltryptamine). Psilocin is far less stable: its phenolic hydroxyl group oxidises easily, so reporting both compounds together gives a more complete picture of the sample.
  • Total alkaloids – the sum of the tryptamine alkaloids detected in the sample, which can include minor related compounds besides psilocybin and psilocin.

Expressing these values as quantitative data allows you to compare strains, flushes and batches on an objective basis and to document your research reproducibly.

Why does alkaloid content vary?

Alkaloid concentration shows considerable variability, not only between species and strains but also between fruiting bodies from the same culture. Several factors contribute to it:

Genetics and biosynthesis

Psilocybin is synthesised from the amino acid L-tryptophan through a short enzymatic pathway encoded by a cluster of genes (psiD, psiH, psiK and psiM). Differences in genetic background and gene expression lead to different alkaloid profiles, which is why strains such as Tidal Wave Ape, Albino Jedi Mind Fuck, Cascadian Teacher or Whitebilly need to be compared with measured data.

Cultivation conditions and flush

Substrate composition, temperature, humidity, light and the developmental stage at harvest all influence the metabolism of the fungus. Values may also change between the first and subsequent flushes, so each harvest should be treated as a separate data point.

Post-harvest handling

Psilocin degrades readily in the presence of oxygen, light, heat and moisture, and psilocybin can be dephosphorylated to psilocin when tissue is damaged. Drying method, storage time and storage conditions therefore have a direct effect on the values measured in the laboratory.

Blue bruising is not a potency indicator

The blue colour that appears when the tissue is damaged is produced by the enzymatic oxidation of psilocin into blue oligomers. It indicates that psilocin is being degraded, not how much psilocybin the sample contains, so it cannot replace an analytical measurement.

Sample preparation guidelines

The reliability of any analysis starts with the sample. To obtain a representative result and minimise degradation before analysis:

  • Dry the material completely before sending it. Residual moisture favours enzymatic and oxidative degradation during transit.
  • Avoid high drying temperatures, which accelerate the breakdown of psilocin.
  • Minimise storage time between drying and shipping. Alkaloid content decreases progressively over time.
  • Protect the sample from light, heat and humidity until it is posted.
  • Seal it in the MycoTest Bag to limit exposure to air and physical damage.
  • Label each sample with its unique code and use one code per sample. To compare strains, flushes or drying methods, send each condition as a separate MycoTest.

Analyse and compare our varieties

Our grow kits use selected genetics by Full Canopy. Combine them with MycoTest to quantify and compare the tryptamine profile of each strain. See how they differ in our guide to magic mushroom strains.

Frequently asked questions

You will receive your quantitative report by email in less than 3 weeks.

MycoTest costs €25 per sample. The price includes the laboratory analysis and the MycoTest Bag with its pre-printed envelope, everything you need to send your sample.

Each report quantifies three values for your sample: psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine), PCBE (psilocybin + psilocin) and total tryptamine alkaloids.

No. The blue colour is produced by the enzymatic oxidation of psilocin into blue oligomers. It shows that psilocin is being degraded, but it does not indicate how much psilocybin or total alkaloid the sample contains. Only an analytical measurement can quantify it.

Yes. Each sample is identified only by the unique code you receive with your purchase, and the report is sent to the email address linked to your order.

Write your unique code on the envelope, place the sample inside the MycoTest Bag and post it using the pre-printed envelope. You do not need to write any address.

Yes. MycoTest can analyse samples from any variety, including Full Canopy genetics such as Tidal Wave Ape, Albino Jedi Mind Fuck, Cascadian Teacher and Whitebilly, which makes it useful for comparing alkaloid profiles between strains.

Alkaloid content depends on genetics, cultivation conditions, developmental stage at harvest, flush and post-harvest handling. Natural biological variability means that fruiting bodies from the same culture can give different results.

Yes. Psilocin is sensitive to oxygen, light, heat and moisture, and psilocybin can be converted to psilocin when tissue is damaged. For the most representative result, send a fully dried sample as soon as possible after drying.

Yes. Send each strain or flush as a separate MycoTest, each with its own unique code. You will receive one report per sample, so you can compare the values side by side.

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